1 My Apollo window has frozen. What do I do?
2 Some data tracks are very slow to load.
3 I used to see tracks and now they are gone.
4 What is the meaning of the lower case letters in the nucleotide sequence?
5 Is it possible to know which organism is the source for a particular protein alignment (in the Protein Alignment track)?
6 Where is the track metadata?
7 What does the warning symbol mean?
8 The track I've viewing says 'Max height reached' but I want to see more rows. How do I do that?
9 How do I see genes that need secondary review?
10 I want to drag an element from an evidence track to the user-created annotation track but I can't!
11 Comment/Renaming-related questions
12 What do those clear/all white gene models mean? Should we keep them?
13 How does secondary review work?
14 Apollo automatically changed the ORF when I dragged the gene model to the user created annotations band. How do I fix it?

My Apollo window has frozen. What do I do?

Try reloading your browser window.

For Chrome: Shift Command R
For Firefox: Ctrl+R
For Safari: shift+reload

Some data tracks are very slow to load.

The way JBrowse works within Apollo, the first time your load up a track it might take a while. But once loaded, any successive loads will be quicker.

I used to see tracks and now they are gone.

Close the track and open it again. Yes, this is the standard 'turn it off and turn it on again' solution.

What is the meaning of the lower case letters in the nucleotide sequence?

These indicate regions of low complexity. They have no impact/implication with respect to annotation.

Is it possible to know which organism is the source for a particular protein alignment (in the Protein Alignment track)?

By right clicking on the alignment, and then selecting 'View details.' You'll see the GenBank accession in the id section and can search for that at NCBI.

Where is the track metadata?

TRACK NAME	Description of data
Col-CC_Genomic_Annotations_Data	Result of NCBI Eukaryotic Annotation Pipeline
AT-Col-CC-Liftoff-from-TAIR10.1	v11 models mapped to v12 reference using Liftoff
TranscriptomeReconstructoR models	Method: (1) Assembled expression evidence: ONT-DRS (ERR3764345 - ERR3764351), CAGE-Seq (SRR10045003 - SRR10045005), PAT-Seq (SRR7160296, SRR7160297, SRR7160299), plaNET-Seq (SRR9117170 - SRR9117173) (2) Aligned all the datasets to Col-CC genome (3) Built TRR based annotation using alignment (bam) files Output: High Confidence (HC) genes - full length mRNA picked from ONT which have both proper start site from CAGE and end site from PAT-Seq coinciding with ONT data. Medium Confidence (MC) genes - full length mRNA picked from ONT with either start site from CAGE or end site from PAT-Seq not coinciding with ONT data. Low Confidence (LC) genes - full length mRNA picked from ONT with both start site from CAGE and end site from PAT-Seq not coinciding with ONT data. Single Method Confidence (SMC) genes - novel genes annotated only from plaNET-Seq data.
Gnomon Models	One of the outputs of the annotation pipeline. These are a superset of the final set of annotated models. "Gnomon annotation of the genomic sequence. Sequence identifiers are provided as accession.version for the genomic sequences and Gnomon identifiers for the Gnomon models:gene.XXX for genes, GNOMON.XXX.m for transcripts and GNOMON.XXX.p for proteins. These identifiers are NOT universally unique. They are unique per annotation release only." (from NCBI documentation)
Coverage Tracks, RNAseq and Long Read
RNAseq capped_merged_filtered coverage	Coverage track version of the RNAseq capped_merged_filtered reads.
Long Read Alignments coverage, plus strand	Coverage track of the long reads, plus strand ONLY.
Long Read Alignments coverage, minus strand	Coverage track of the long reads, minus strand ONLY.
Merged, Capped, and Filtered RNAseq Reads
RNAseq capped_merged_filtered reads	RNAseq reads from the 62 RNAseq experiments after filtering for overly long (>5Kbp) inserts, and successive capping and merging to try to reduce the overabundance of highly expressed genes. File was then filtered to remove overly long (>12Kbp) introns.
Protein Evidence
PFAM domains	Results from an INTERPROSCAN run on the proteins from the V12 prediction to get the PFAM domain information, converted to absolute position on the Col-CC assembly.
PFAM domains - Liftoff	Results from an INTERPROSCAN run on the proteins from the Araport11 release to get the PFAM domain information, converted to absolute position on the Col-CC assembly (using the Liftoff file that converted Araport11 coordinates to Col-CC coordinates).
PANTHER families	Results from an INTERPROSCAN run on the proteins from the V12 prediction to get the PANTHER family information, converted to absolute position on the Col-CC assembly.
PANTHER families - Liftoff	Results from an INTERPROSCAN run on the proteins from the Araport11 release to get the PANTHER family information, converted to absolute position on the Col-CC assembly (using the Liftoff file that converted Araport11 coordinates to Col-CC coordinates).
Protein alignments chained	Alignments of Arabidopsis thaliana and other Brassicaceae proteins, including Araport 11 annotated proteins, to the genomic sequence(s). These alignments may have been used as evidence for gene prediction by the NCBI annotation pipeline. Pieces of the same protein have been connected together for easier visualization.
TRR CAGE PAT plaNET
CAGE forward	TranscriptomeReconstructoR models CAGE evidence, forward strand (evidence supporting the start site of transcription)
CAGE reverse	TranscriptomeReconstructoR models CAGE evidence, reverse strand (evidence supporting the start site of transcription)
PAT forward	TranscriptomeReconstructoR models PAT evidence, forward strand (evidence supporting the end site of transcription)
PAT reverse	TranscriptomeReconstructoR models PAT evidence, reverse strand (evidence supporting the end site of transcription)
plaNET forward	TranscriptomeReconstructoR models plaNET evidence, forward strand (evidence supporting transcription of mRNAs and lncRNAs)
plaNET reverse	TranscriptomeReconstructoR models plaNET evidence, reverse strand (evidence supporting transcription of mRNAs and lncRNAs)
Transcript Evidence
Known Reference Sequences	"Alignments of the annotated Known RefSeq transcripts (identified with accessions prefixed with NM_ and NR_) to the genome." (from NCBI documentation) These were NOT used in generating the Col-CC annotation. They are alignments of the annotated transcripts to the genome and can provide additional insight into the predicted gene structures independent of the prediction.
Model Reference Sequences	"Alignments of the annotated Model RefSeq transcripts (identified with accessions prefixed with XM_ and XR_) to the genome." (from NCBI documentation) These were NOT used in generating the Col-CC annotation. They are alignments of the annotated transcripts to the genome and can provide additional insight into the predicted gene structures independent of the prediction.
Col-CC Same Species Combined	Alignments of same-species cDNAs, ESTs and TSAs to the genomic sequence(s). cDNAs and ESTs alignments (not TSAs) may have used as evidence for gene prediction by the NCBI annotation pipeline. The TSA alignment track is a subset of the Col-CC Same Species track. Pieces of the same transcript have been connected together for easier visualization.
TSA alignment	Alignments of transcripts assembled from RNA-Seq reads, and submitted to GenBank (see accessions DAHAIV01, GGJX01, GJRK01 and GKIF01). These were not used as evidence for gene prediction by the NCBI annotation pipeline.
RNA seq tracks from various plant parts and growth stages/conditions of those parts	Name is based on the GenBank record, for example, SRR1019221. You can link to that record using this base URL for more information on the experiment: https://www.ncbi.nlm.nih.gov/sra/SRR1019221
Long Read alignments	Alignments of individual IsoSeq reads in SRA. These alignments may have been used as evidence for gene prediction by the NCBI annotation pipeline. Right clicking on the read itself will allow you to ‘View Details’ and see the ID of the SRA entry for the experiment. Using the id (e.g., SRR11031292), you can go to the full GenBank record for the experiment. https://www.ncbi.nlm.nih.gov/sra/?term=SRR11031292.