2025 Updates

2025 Updates

Date

Update

Date

Update

Feb. 11, 2026

ENA record partially released (noncoding metadata only). Following up with ENA about the rest of the data.

Feb. 4, 2026

File submitted successfully to ENA. Under final review.

Jan. 28, 2026

ENA made requests, they are being processed. Genbank pointed out some possible fixes, will be incorporated into ENA final files. Working on JBrowse2 that will show new TAIR12 tracks.

Jan. 21, 2026

ENA is still trying to get the TAIR12 data through their pipeline. No further action possible or needed on our end. No estimate of completion data yet.

Jan. 14, 2026

No updates since the last report. We’ll post here as soon as anything changes.

Jan. 7, 2026

Nothing new to report. As soon as we know anything new, we’ll post here.

Dec. 29, 2025

Holiday break at ENA. Nothing new to report.

Dec. 17, 2025

Nothing new to report. Waiting for ENA to respond on remaining issues.

Dec. 10, 2025

Nothing new to report.

Dec. 3, 2025

Working with ENA for last remaining validation issues. Our data is good but their software is incorrectly flagging some cases of exon overlaps.

Nov. 26, 2025

Positive conference call with ENA and NCBI - submission will go to ENA and will flow into NCBI in time. webincli shows one last error that needs to be resolved by manual intervention.

Nov. 19, 2025

ENA and GenBank discussions in progress

Nov. 12, 2025

ENA submission: stalled, ENA is backed up https://www.ebi.ac.uk/ena/browser/support

“Kindly note that the ENA helpdesk response time is approximately 3 weeks due to the current volume of tickets. We apologise for this delay and thank you for your patience.

Due to a very high increase in recent submissions of a specific type of data to ENA in recent months we are experiencing a delay in processing and data exchange with INSDC partners. Processing and display of ENA assemblies should return to normal shortly. However we are unable to give a timeline when these will complete full exchange with INSDC partners. Thank you in advance for your patience. We will notify users again when we believe service is restored to normal.”

Nov. 5, 2025

ENA Submission: all files are uploaded into ENA and are being reviewed one last time by ENA’s lead bioinformatician, awaiting final feedback, release date pushed to Nov. 7.

Oct. 29, 2025

ENA Submission in progress: final internal validation run ongoing, minimal updates required, GFF and FASTA files to be submitted by webin-cli after clean run

Oct. 22, 2025

ENA Submission in progress: assembly and annotation to be uploaded in the next week with an anticipated release date of Nov. 3, 2025.

Oct. 15, 2025

Set up for submission of genome assembly and annotation through the European Nucleotide Archive (ENA).

Oct. 8, 2025

Exploring alternatives to GenBank for annotation submission.

Oct. 1, 2025

GenBank submission: working through last warnings of discrepancy report

US government shutdown does affect the NCBI/NIH.

“Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted.” - Source: NCBI website

Sept. 24, 2025

GenBank submission: updated file was submitted, table2asn checking script was updated on 9/16/25, now additional errors and warnings are showing up, under evaluation

Sept. 18, 2025

GenBank submission: ability to upload an updated submission file added, official review of initial submission received

Sept. 17, 2025

GenBank submission: still in processing phase

Manuscript: last trickles

Aug. 27, 2025

GenBank submission: slow but steady progress

Manuscript: last updates trickling in

Aug. 20, 2025

GenBank submission: Back to work.

Aug. 13, 2025

GenBank submission: Still holding. (thank you for understanding)

Aug. 6, 2025

GenBank submission: Holding. (medical emergency)

July 31, 2025

Deadline: last chance to edit text and figures for manuscript.

July 30, 2025

Contact reestablished at NCBI! TE issue brought to light, recalculating.

July 23, 2025

Still waiting for response from NCBI. Reaching out to additional contact there.

July 16, 2025

TE minor updates incorporated into main file. Waiting for response from NCBI.

July 9, 2025

Update from TE team received on 7/4. Contact reestablished with NCBI.

July 4, 2025

Deadline: last chance for sending minor changes to GFF to Alyssa.

July 2, 2025

Waiting to upload next annotation file iteration to GenBank, need directory information. Waiting for last minor GFF changes.

June 27, 2025

Manuscript finalization call.

Decision: release TAIR12 via GenBank as soon as all quality control checks have passed.

June 19, 2025

Arabidopsis Informatics workshop at ICAR2025

June 18, 2025

Still waiting for NCBI staff member to return to office and respond. Will shift to secondary contact.

June 17-20, 2025

ICAR2025 in Ghent, Belgium - looking forward to seeing people there.

June 11, 2025

Most Genbank issues resolved, waiting for NCBI staff member to return to office.

June 4, 2025

Still working through Genbank submission issues, mostly have to do with ncRNA formatting.

May 22, 2025

Working on addressing issues with GenBank submission. Various groups working on their parts of the manuscript: text and figures.

May 14, 2025

Call planned for end of June to finalize manuscript. Collection of final figures and text in the time between now and then.

May 8, 2025

Follow up and clarification email sent to GenBank. The staff member assisting us is out of office until May 14, 2025.

May 7, 2025

Initial comments received. No blocking errors. Warnings have been prioritized. Remediation in progress.

May 2, 2025

Reminder email sent out to all groups contributing to the manuscript.

April 28, 2025

Annotation submitted to GenBank. Finally. Now, we await review and acceptance.

April 23, 2025

Two ERRORS left to clear: cDNA/mRNA mismatch and one small intron

Will submit to portal after these are cleared and see what next steps are.

April 17, 2025

  1. IN PROGRESS submission to GenBank, under review with NCBI staff

    1. minor issues all addressed, waiting for reply fromGenBank on rRNA formatting

April 9, 2025

  1. IN PROGRESS submission to GenBank, under review with NCBI staff

    1. current validation run shows single digits of errors per chromosome for most categories

April 2, 2025

  1. IN PROGRESS submission to GenBank, under review with NCBI staff

    1. table2asn errors cleaned up significantly

Mar. 26, 2025

  1. DONE Adjust FASTA headers for protein submission

  2. IDONE Run checks to make sure there are no duplicate features or other strange formatting in col9

  3. DONE iterative local runs of table2asn, review and addressing of errors

  4. IN PROGRESS submission to GenBank, under review with NCBI staff - 12 categories detected by manual inspection

Mar. 19, 2025

  1. IN PROGRESS Adjust FASTA headers for protein submission

  2. IN PROGRESS Run checks to make sure there are no duplicate features or other strange formatting in col9

  3. DONE parent feature for TE mobile elements

  4. IN PROGRESS iterative local runs of table2asn, review and addressing of errors

  5. No more outside requests for changes

  6. Scheduled for March 24 submission to GenBank

Mar. 12, 2025

  1. DONE duplicate '/product information from the mRNA to the matching CDS lines

  2. DONE verify all col3 features

  3. DONE miRNA annotations: deconvolute pre-miRNA, miRNA_primary_transcript, miRNA

  4. NOT YET STARTED Adjust FASTA headers for protein submission

  5. DONE Fix several cases of exons extending past parent feature

  6. IN PROGRESS Run a check to make sure there are no duplicate features in col9

  7. IN PROGRESS: Should be done by the end of this week parent feature for TE mobile elements

Mar. 4, 2025

  1. DONE duplicate '/product information from the mRNA to the matching CDS lines

  2. DONE verify all col3 features

  3. IN PROGRESS: ‘derives from’ feature being added: miRNA annotations: deconvolute pre-miRNA, miRNA_primary_transcript, miRNA

  4. NOT YET STARTED Adjust FASTA headers for protein submission

  5. DONE Fix several cases of exons extending past parent feature

  6. IN PROGRESS Run a check to make sure there are no duplicate features in col9

  7. IN PROGRESS: New file incorporated, final check on numbering left to do parent feature for TE mobile elements

Feb. 26, 2025

  1. DONE duplicate '/product information from the mRNA to the matching CDS lines

  2. DONE verify all col3 features

  3. IN PROGRESS: New rules developed based on discussion with lncRNA group: miRNA annotations: deconvolute pre-miRNA, miRNA_primary_transcript, miRNA

  4. NOT YET STARTED Adjust FASTA headers for protein submission

  5. DONE Fix several cases of exons extending past parent feature

  6. IN PROGRESS Run a check to make sure there are no duplicate features in col9

  7. IN PROGRESS: New file provided by TE group needs evaluation (Pseudo code, but have not implemented) parent feature for TE mobile elements

February 13, 2025

Still not there.

Remaining prep of GFF file for GenBank submission:

  1. duplicate '/product information from the mRNA to the matching CDS lines

  2. verify all col3 features

  3. miRNA annotations: deconvolute pre-miRNA, miRNA_primary_transcript, miRNA

  4. Adjust FASTA headers for protein submission

  5. Fix several cases of exons extending past parent feature

  6. Run a check to make sure there are no duplicate features in col9

  7. (Pseudo code, but have not implemented) parent feature for TE mobile elements

January 2025

Goal: Manuscript draft complete.

January 2025

Goal: submission to GenBank complete.

January 13, 2025

TAIR talk at Arabidopsis Informatics session at PAG32, with section on TAIR12

Slides available

January 6, 2025

GFF3 files sent out to annotation teams for final review.