Chromosome 1

Clone F8L2

CEN1: 3 asm; 3 grps. Will most likely close but mainly Athila etc. Priority 3.

Clone F12G6

GenBank PLN, centromeric, 3 unordered (SSP)

Clone T28N5

GenBank PLN, centromeric, 11 unordered (SSP)

Clone F25O15

CEN1: 2 asm; 1 grp. This BAC should close soon. Priority 3.

Clone F9D18

GenBank PLN, centromeric, contains gap of approx. 34 kb (SSP)

Clone T2P3

Euchr 1: ~20 asm; 10 grps. Contains >99% identical repeats. We have made many different libraries for this BAC in attempts to get more uniform coverage. Two ~30 kb and ~ 35 kb assemblies that overlap the neighboring BACs have been annotated to assess their gene content. Transposon-based sequencing of linking clones continues. We expect to be able to reduce the number of assemblies and annotate then even if full closure is not possible. Priority 1.

Chromosome 2

Clone T6C20

CEN2: This BAC lies within the 180 bp repeats; thus full closure was not attempted in 1998-1999 when chromosome 2 was completed. We have revisited this BAC, including making a large insert library, but find that this does not help. This BAC will not be closed. Priority 4.

Clone F10C8

Chromosome 3

Clone T25F15

CEN3: This BAC lies in one of two small island contigs within CEN3 and contains 180 bp repeats. It is in 2 asm, 1 grp with one apparent sequencing gap. Nested deletions of a spanning clone have been made that should in theory close this BAC and are in the sequencing queue. Priority 3.

Clone T28G19

CEN3: 22 asms; 10 grps even when edited to 6+ and assembled at 100%. This BAC lies in one of two small island contigs within CEN3. Most likely the status of this BAC cannot be improved by further effort. Priority 4.

Clone T18B3

CEN3: 8 asms; 4 grps. We can make progress with this BAC and will do so. Priority 3.

Clone F21A14

CEN3: 26 asms; 3 grps after editing and assembly at 100%. This BAC cannot be closed. Priority 4.

Chromosome 4

Clone F26K10

HTG (Sanger)

Clone T5F17

HTG (Sanger)

Clone F17A13

HTG (Sanger)

Clone F6E21

HTG (Sanger)

Clone T16I18

HTG (Sanger)

Chromosome 5

Clone T19N18

No GenBank record (Sanger?) TIGR has annotated sequence for this clone.

Clone T26N4

CSHL finishing (includes T22F2). Contains 5S rRNA repeats.

Clone T25B21

CSHL finishing, contains 5S rRNA repeats.

Unanchored Clones

F26J21

65 asms; 32 gps (but most sequences fall into 5 asms, 2 grps). Appears to contain many genes. We plan to close and resolve as much as possible. Awaiting more sequence. Priority 2.

T13I7

21 asms; 9 grps. We plan to close and resolve as much as possible. Awaiting large insert library and more sequence. Priority 2.

T4N13

52 asms; 37 grps. We plan to close and resolve as much as possible. Awaiting large insert library and more sequence. Priority 2.

T17J15

26 asms; 14 grps. We plan to close and resolve as much as possible. Awaiting large insert library and more sequence. Priority 2.